SPECTROPHOTOMETRIC ESTIMATION FOR SIMULTANEOUS ESTIMATION OF TINIDAZOLE AND DILOXANIDE FUROATE USING HYDROTROPIC AGENTS

Abstract

A simple, accurate, and precise spectrophotometric method was developed for the simultaneous
estimation of Tinidazole (TDZ) and Diloxanide Furoate (DXF) using hydrotropic agents. The poor
aqueous solubility of both drugs was enhanced using a combination of 2M Sodium citrate and 8M Urea
(1:1), which provided stability, solubility, and minimal spectral interference. TDZ and DXF showed
λmax at 291.0 nm and 257.0 nm, respectively, with distinct absorption spectra allowing simultaneous
analysis. Linearity was observed in the concentration range of 5–25 μg/mL with regression equations Y
= 0.056X – 0.0067 (R² = 0.9997) for TDZ and Y = 0.0486X + 0.0045 (R² = 0.999) for DXF. Recovery
studies at 80%, 100%, and 120% levels yielded mean recoveries of 98.66–98.89% for TDZ and 98.88–
98.99% for DXF with %RSD < 1, indicating high accuracy. Validation parameters, including
repeatability, day-to-day variation, analyst-to-analyst variation, and reproducibility, confirmed precision
and robustness. Application of the method to marketed tablet formulations demonstrated label claim
compliance with low %RSD values. The proposed method is rapid, cost-effective, and suitable for
routine quality control of TDZ and DXF in combination formulations.