FORMULATION AND EVALUATION OF SEMISYNTHETIC ANTIFUNGAL INSITU GEL CONTAINING SENNA AURICULATA AND AZADIRACHTA INDICA LEAVES
Keywords:
Senna auriculata,, Neem (Azadirachta indica), In-situ Gel,, Antifungal Activity, Carbopol 934,, Topical Formulation.Abstract
The antimicrobial in-situ gel containing extracts of Senna auriculata flower and Azadirachta indica
leaves for topical drug delivery. Herbal medicines are widely used for the treatment of skin infections
and inflammatory conditions due to their therapeutic effectiveness, safety, easy availability.
The prepared formulations were evaluated for various physicochemical parameters including
appearance, color, odor, homogeneity, pH, viscosity, spread ability, gelation capacity. Spread ability
was determined to evaluate ease of application, while viscosity studies were performed using Brookfield
viscometer to determine rheological behavior of the gel. The pH of the formulation was maintained
within the range suitable for skin application. The antimicrobial activity of the prepared in-situ gel was
evaluated by agar well diffusion method against selected microorganisms including Staphylococcus
aureus, Escherichia coli, and Candida albicans. The optimized formulation exhibited significant
antimicrobial activity with satisfactory zone of inhibition against test organisms. Stability studies were
carried out under different storage conditions to evaluate physical stability, pH, viscosity, and
homogeneity of the formulation. The results of the study demonstrated that the formulated in-situ gel
showed satisfactory physicochemical characteristics, good spread ability, and acceptable viscosity,
excellent and significant antimicrobial activity.
