GC-MS Studies in Ethyl acetate extract of Rare, Endemic Medicinal plant Crotalaria ramosissima - A Comparative Study

Abstract

Gas chromatography mass spectrometry (GC-MS) analysis was carried out to investigate the
phytochemical composition of the stem and root of Crotalaria ramosissima. The study revealed the
presence of a diverse range of bioactive compounds, with 28 compounds identified in the stem ethyl
acetate extract and 30 compounds detected in the root ethyl acetate extract. These compounds belong to
various chemical classes such as alkaloids, flavonoids, terpenoids, fatty acids, and phenolic derivatives,
which are known for their significant pharmacological activities. Many of the identified constituents
exhibit potent antimicrobial, antioxidant and anti-inflammatory properties, suggesting their potential role
in therapeutic applications. The variation in compound distribution between stem and root indicates
differential biosynthesis and accumulation of metabolites in plant parts. The presence of these bioactive
molecules supports the traditional medicinal use of Crotalaria ramosissima and highlights its importance
as a promising source of natural drugs. This study provides a scientific basis for further investigation
into the isolation, characterization, and biological evaluation of these compounds. Overall, the GC-MS
profiling emphasizes the plant’s potential in pharmaceutical and nutraceutical development.